What this entity is
Canonical name: Acetate. 1
Known aliases: No additional aliases are reported in this document. 1
Stable external identifier: None is reported in this document. 1
Key facts
Acetate was one of the 8 carbon sources tested in the Acinetobacter baylyi ADP1 deletion-collection growth matrix, which measured 2,034 genes. 1
Acetate belongs to the demanding condition tier, together with urea and butanediol; these conditions had mean growth ratios of 0.41–0.65 and 95–100% of genes showing defects at a growth ratio below 0.8. 1 Growth ratios were calculated as mutant/wild-type values, with values below 1.0 indicating growth defects. 1
Among the 28 condition pairs, acetate–butanediol had the highest Pearson correlation, at r = 0.58, indicating that these two demanding conditions shared the strongest measured association in the matrix. 1
Acetate-specific genes included fadB, malate synthase G, and citB, mapping acetate utilization to fatty acid β-oxidation and the glyoxylate-shunt. 1 The acetate-associated phenotype therefore contributes to the report’s broader finding that gene importance varies continuously with the growth condition rather than being fully captured by binary essentiality labels. 1
The aromatic-catabolism analysis refines acetate’s role as a demanding comparison condition: acetate produced the largest reported Complex I fitness defect relative to background, -1.55, and is interpreted as a non-aromatic substrate that generates high NADH flux through the TCA cycle. 2 This result supports the hypothesis that Complex I dependence tracks NADH-generating substrate use rather than aromatic catabolism alone; across the transferred dataset, Complex I orthologs had mean fitness values of -1.35 on aromatic conditions versus -0.77 on comparison conditions, with Mann-Whitney p < 0.0001. 2
The respiratory-chain analysis further refines this interpretation: acetate requires Complex I, cytochrome bo3, ACIAD3522, and additional respiratory components, with no listed component dispensable in the reported profile. 3 Acetate generated 3 NADH, or 1.50 NADH per carbon, with production assigned to the TCA cycle; Complex I had a growth ratio of 0.49, while ACIAD3522 had a growth ratio of 0.013 and was described as lethal in this condition. 3 These findings support a condition-specific respiratory configuration driven by NADH flux and capacity constraints, while the theoretical stoichiometry does not constitute a measured flux distribution. 3
Related pages
The acetate findings feed into condition-specific-fitness because they quantify carbon-source-dependent growth requirements and refine the distinction between demanding-condition phenotypes and high-NADH-flux respiratory dependence. 1,2,3
They also refine gene-essentiality by showing that deletion effects depend on the tested condition. 1
The respiratory result supports metabolic-model-gapfilling because growth-optimized FBA routes NADH through the ATP-favorable pathway and can miss alternative condition-specific respiratory requirements. 3
The complete deletion-phenotype analysis is summarized in ADP1 Deletion Collection Phenotype Analysis. 1
The aromatic respiratory interpretation is detailed in Aromatic Catabolism Support Network in ADP1. 2
The condition-specific respiratory-chain analysis is detailed in Condition-Specific Respiratory Chain Wiring in ADP1. 3