What this entity is
Canonical name: Pseudomonas stutzeri RCH2. 1
Known alias: psRCH2. 1
No stable external identifier for this strain is provided in the source document. 1
Key facts
Pseudomonas stutzeri RCH2 is an outlier in the cross-organism analysis of genes that are both costly in laboratory fitness measurements and dispensable in the pangenome. 1 It contributes 21.5% of its genes to the costly+dispensable category, compared with 14.0% for the next organism, bacteroides-thetaiotaomicron. 1
The report interprets this unusually high proportion as potentially reflecting a recent mobile-element invasion or strain-specific genomic expansion, but the cause remains unresolved. 1 This strain-level outlier contributes to the broader pangenome-integration finding that laboratory fitness burden and pangenome conservation can identify evolutionarily unstable gene sets. 1 It also informs gene-essentiality by illustrating that costly genes need not be conserved across the pangenome. 1
The new functional-dark-matter analysis refines this outlier context by including P. stutzeri among organisms with 45 GapMind-gapped pathways and by selecting a P. stutzeri RCH2 stress experiment in its ten-experiment campaign. 2 These results support further condition-specific investigation but do not establish that the strain’s costly+dispensable genes cause the pathway gaps. 2
The pathway-capability analysis further supports condition-specific investigation by listing P. stutzeri RCH2 among the seven Tier 1 organisms used to compare GapMind pathway completeness with Fitness Browser fitness data; only 7 of 48 Fitness Browser organisms had matching GapMind genome data. 3 This inclusion establishes comparative data coverage for the strain, but does not attribute any particular Active Dependency, Latent Capability, Incomplete but Important, or Missing classification to RCH2 in the report. 3
The source cautions that the 21.5% proportion may reflect strain-specific genomic features rather than a general pattern. 1
Related source
The full costly-dispensable-gene analysis is summarized in The 5,526 Costly + Dispensable Genes. 1 The functional-dark-matter integration is summarized in Functional Dark Matter — Experimentally Prioritized Novel Genetic Systems. 2 The pathway-capability analysis is summarized in Metabolic Capability vs Dependency. 3