Identity
Lactate is the canonical compound name used for a carbon source in the Acinetobacter baylyi ADP1 deletion-phenotype analysis. 1
- Canonical name: Lactate. 1
- Known aliases: No aliases were reported in the source document. 1
- Stable external identifier: No stable external identifier was reported in the source document. 1
Evidence from ADP1 and cross-collection integration
Lactate was one of 8 carbon sources tested across a complete growth matrix containing 2,034 genes. 1
Lactate belonged to the moderate condition tier, together with asparagine; these conditions had mean growth ratios of 0.80–0.82 and 37–45% of genes showing defects. 1
The lactate-specific gene pattern included lldR and cyoC/cyoD, linking lactate-dependent growth phenotypes to lactate utilization and respiratory functions. 1
The respiratory-chain analysis refines this claim by identifying cytochrome bo3 as specifically required for lactate growth, while cytochrome bd is dispensable and Complex I is only mildly important; the reported Complex I growth ratio is 0.77. 2
The respiratory analysis further reports 5 NADH produced from lactate, or 1.67 NADH per carbon, with production through pyruvate plus the TCA cycle; this theoretical stoichiometry suggests that lactate selects a distinct respiratory configuration rather than simply a quantitative level of respiratory demand. 2
A separate Web of Microbes (WoM) analysis supports the value of lactate as a cross-collection phenotype: lactate was an E (“emerged”) metabolite for Pseudomonas sp. FW300-N2E3, meaning it was absent from the starting medium and newly detected, and Sodium D-Lactate was tested as a carbon source in the Fitness Browser. 3 This bridge does not establish the ADP1 respiratory mechanism, but it provides a direct metabolite-production-to-fitness comparison that is adjacent to the ADP1 condition-specific result. 3
These findings support condition-specific-fitness by showing that lactate exposes an intermediate level of condition-dependent deletion sensitivity rather than the extreme sensitivity observed under demanding conditions or the low defect rate observed under robust conditions. 1
They also refine gene-essentiality because lactate-associated growth effects illustrate that gene importance can vary by growth condition rather than fitting a strictly binary essential/non-essential classification. 1
The source analyses integrate deletion growth measurements with TnSeq classifications, functional annotations, pangenome status, respiratory-chain fitness, FBA, theoretical stoichiometry, cross-species comparisons, and proteomics, connecting lactate phenotypes to multi-omics-integration. 1 2
Source
- ADP1 Deletion Collection Phenotype Analysis — Summary of the ADP1 deletion collection phenotype analysis, including lactate condition classification and associated genes. 1
- Condition-Specific Respiratory Chain Wiring in ADP1 — Condition-specific respiratory-chain wiring analysis for ADP1, including lactate-associated cytochrome bo3 dependence. 2
- Web of Microbes Data Explorer — WoM metabolite-action semantics and links between lactate production and Fitness Browser carbon-source experiments. 3