What this entity is
Canonical name: Entner-Doudorff pathway. 1
Known aliases: None reported in this document. 1
Stable external identifier: None reported in this document. 1
The Entner-Doudorff pathway is a glucose-associated metabolic pathway identified in the ADP1 deletion-phenotype analysis. 1
Evidence from ADP1 deletion phenotypes
The complete growth matrix measured 2,034 genes across 8 carbon sources, and glucose-specific phenotypes selected eda, gntT, gluconokinase, and glucose dehydrogenase (PQQ) as genes associated with the Entner-Doudorff pathway and PQQ-dependent glucose utilization. 1
The report identifies glucose as one of three robust conditions, alongside glucarate and quinate, with mean growth ratios of 1.25–1.36 and 0.5–2.4% of genes defective at the growth-ratio threshold of less than 0.8%. 1
PQQ biosynthesis genes were condition-specific for both glucose and quinate, consistent with PQQ-dependent dehydrogenases catalyzing the first step of both pathways. 1
Across all eight carbon sources, 625 genes, representing 31% of the complete matrix, had a condition-specificity score of at least 1.0. 1
Respiratory-chain interpretation
The respiratory-chain analysis refines the pathway’s glucose association by proposing that glucose distributes NADH production across Entner-Doudoroff pathway steps and the TCA cycle, generating 9 total NADH, or 1.50 NADH per carbon. 2
This distributed production is proposed to remain within the capacity of NDH-2, helping explain why no specifically required respiratory component was detected on glucose and why Complex I had a growth ratio of 1.44 there. This is a theoretical flux interpretation rather than a measured flux distribution. 2
The same analysis contrasts glucose with quinate: quinate-associated aromatic-ring cleavage is proposed to create a concentrated TCA-cycle NADH burst, despite producing 4 total NADH, or 0.57 NADH per carbon, making Complex I more important on quinate. 2
Related pages
- glucose — glucose was the condition associated with Entner-Doudoroff pathway genes in the deletion analysis. 1
- pqq-biosynthesis — PQQ biosynthesis genes were condition-specific for glucose and quinate. 1
- condition-specific-fitness — the pathway is evidence that gene importance depends on the tested carbon source. 1
- ADP1 Deletion Collection Phenotype Analysis — source summary for the ADP1 deletion collection phenotype analysis. 1
- Condition-Specific Respiratory Chain Wiring in ADP1 — source summary for the condition-specific respiratory-chain interpretation. 2