Identity
Canonical name: ManXYZ. Known alias: ManX-family PTS domain. Stable external identifier: No stable external identifier was reported in the source. 1
ManXYZ is a phosphotransferase-system transporter associated with mannose and glucosamine uptake. 1 The new SNIPE analysis supports this assignment and contradicts a fructose-specific interpretation: in 168 Escherichia coli K-12 experiments, manX, manY, and manZ showed fitness values of -2.75, -3.00, and -2.74 on D-mannose, versus -0.22, +0.15, and -0.07 on D-fructose; fruA was -0.06 on D-mannose and -1.44 on D-fructose. 2
Evidence from discoveries
Fitness Browser measurements in Escherichia coli K-12 showed that ManXYZ knockouts had a fitness effect of -3.93 on D-mannose and -2.75 on D-glucosamine. 1
The same knockouts had positive fitness effects of +0.18 to +0.66 on D-fructose, contradicting the interpretation that ManXYZ is a fructose transporter. 1 The new measurements refine this substrate comparison: the strongest reported defects were on D-glucosamine, with manX, manY, and manZ values of -3.79, -2.79, and -3.63, and on D-mannose, with values of -2.75, -3.00, and -2.74. 2
ManXYZ’s three genes also showed cofitness correlations of 0.851 for manX↔manZ, 0.705 for manX↔manY, and 0.725 for manY↔manZ, supporting their operation as a single operon. 2 This transporter function is relevant to snipe-defense-system: SNIPE is proposed to cleave phage DNA entering through the ManYZ pore, potentially retaining mannose transport while avoiding the metabolic cost of transporter loss. 2
A full two-domain SNIPE protein with 129 experiments was identified in Methanococcus maripaludis JJ. 1
Klebsiella had 1 DUF4041 annotation across 3 proteins and 4,619 PTS_EIIC annotations, making it the only PhageFoundry species with both SNIPE and the ManX-family PTS domain in this analysis. 1 The new report refines this co-occurrence: it found 28 mannose PTS IID/ManZ proteins and 1 mannose PTS IIA/ManX protein in the corresponding genome browser, but no Klebsiella SNIPE or ManXYZ fitness data were available. 2
Related pages
The transporter evidence comes from the kescience-fitnessbrowser resource and is interpreted using Escherichia coli K-12 data from escherichia-coli. 1
The associated defense-system comparison connects ManXYZ to snipe-defense-system and to the broader environmental-resistome synthesis. 1
See the complete cross-project account in discoveries and SNIPE Defense System in the KBase Data Lakehouse Pangenome. 1,2