Glycine

Identity

Canonical name: Glycine. ₁

Known aliases: No aliases were reported in this document. ₁

Stable external identifier: No stable external identifier was reported in this document. ₁

Evidence from FW300-N2E3

• Glycine increased in the Web of Microbes exometabolome of Pseudomonas FW300-N2E3. ₁
• Glycine was one of 13 metabolites mapped to GapMind, which predicted a complete pathway for FW300-N2E3. ₁
• Glycine also showed growth in Fitness Browser experiments, contributing to the report’s 13/13 agreement between GapMind pathway predictions and Fitness Browser measurements. ₁
• BacDive recorded glycine utilization in 0 of 1 strain, with 1 negative result and low evidentiary confidence because the sample size was n=1. ₁
• The report classifies glycine among four metabolites showing Web of Microbes production but BacDive non-utilization; because only one BacDive measurement was available, it considers this result insufficient for a conclusion. ₁
• The glycine comparison illustrates the distinction between metabolite production measured by exometabolomics and utilization measured by growth assays, which are not intrinsically contradictory. ₁

Community metabolic ecology

Community-scale NMDC–GTDB analysis refines the strain-level GapMind evidence above: glycine biosynthesis showed strong ecosystem-associated differentiation, with a Kruskal–Wallis statistic of H = 92.98 and Benjamini–Hochberg false-discovery-rate-adjusted q = 1.2×10⁻¹⁹. It was the strongest listed amino-acid pathway difference, among 17 of 18 pathways significantly differentiated across ecosystem types. ₂

This community result supports the interpretation that glycine-related metabolic potential varies with ecological context, while not establishing whether the difference reflects habitat physicochemistry, taxonomic composition, or both. ₂

Related context

Glycine contributes evidence to multi-omics-integration through the integration of exometabolomics, Fitness Browser, BacDive, and GapMind data. ₁

Its complete GapMind prediction and observed Fitness Browser growth relate to metabolic-model-gapfilling. ₁

Its production-versus-utilization comparison relates to condition-specific-fitness and cross-tenant-data-bridging. ₁

Its ecosystem-associated pathway differentiation also relates to ecotype-environment-gene-content. ₂

The source reports are summarized at Metabolic Consistency of Pseudomonas FW300-N2E3 Across Four KBase Data Lakehouse Databases and Community Metabolic Ecology via NMDC × Pangenome Integration. _{1 2}