What this entity is
Oak Ridge Field Research Center is the canonical name for the groundwater research site associated with the isolation of Pseudomonas FW300-N2E3. 1
Known aliases: Oak Ridge Field Research Center, Oak Ridge Field Research Centre, and ORFRC; the report does not provide a stable external identifier. 1
The lab–field ecology study supports the site’s role as a testbed for connecting laboratory fitness with environmental community structure: it compared Fitness Browser measurements with 16S amplicon profiles and geochemistry across 108 Oak Ridge groundwater sites. 2
Key facts
Pseudomonas FW300-N2E3 was isolated from groundwater at the Oak Ridge Field Research Center in the context of an ENIGMA SFA project. 1
The report proposes that secretion by a prototrophic organism unable to re-assimilate tryptophan could supply that amino acid to auxotrophic community members in the Oak Ridge groundwater community, although the pathway-level distinction between biosynthetic, catabolic, and regulatory fitness genes remains unresolved because the planned NB04 analysis was deferred. 1
FW300-N2E3 produced or increased 58 metabolites in Web of Microbes exometabolomics data, including increased tryptophan; the report identifies this production-plus-growth-but-no-species-level-catabolism pattern as a hypothesis consistent with cross-feeding or signaling. 1
The report proposes community metabolic modeling of the Oak Ridge groundwater community to test whether tryptophan cross-feeding could explain the ecological role of FW300-N2E3 secretion. 1
The new field study refines this cross-feeding and fitness context by showing that field abundance is not a simple readout of aggregate laboratory metal tolerance: five of 11 tested genera had uranium associations after FDR correction, with both positive and negative directions, while the aggregate tolerance–abundance relationship was only suggestive (Spearman rho=0.503, p=0.095, n=12 genera). 2
Related pages
- Metabolic Consistency of Pseudomonas FW300-N2E3 Across Four KBase Data Lakehouse Databases — source report integrating FW300-N2E3 exometabolomics, fitness, utilization, and pathway data.
- Lab Fitness Predicts Field Ecology at Oak Ridge — field comparison of laboratory fitness, Oak Ridge groundwater communities, and geochemistry.
- pseudomonas-fw300-n2e3 — organism isolated from Oak Ridge Field Research Center groundwater.
- enigma-coral — ENIGMA-related research context.
- multi-omics-integration — cross-database integration used to interpret the FW300-N2E3 data.
- cross-tenant-data-bridging — joining evidence across the KBase Data Lakehouse databases.